Controlled coupling of aminoglycoside antibiotics to proteins for use in homogeneous enzyme immunoassays
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چکیده
منابع مشابه
Quantitative homogeneous enzyme immunoassays for amitriptyline, nortriptyline, imipramine, and desipramine.
We describe specific EMIT homogeneous enzyme immunoassays for amitriptyline, nortriptyline, imipramine, and desipramine in patients' serum samples. Before analysis, an easily performed extraction step involving the use of 500 microL of sample and a 1-mL disposable column eliminates cross-reacting polar metabolites. The range of the standard curve for the first three drugs is 25 to 250 microgram...
متن کاملAminoglycoside Antibiotics
Aminoglycosides are hydrophilic, polycationic, aminecontaining carbohydrates that are usually composed of three to five rings. Most aminoglycosides are either natural products or derivatives of soil actinomycetes. They are often secreted by these actinomycetes as mixtures of closely related compounds. The polycationic aminoglycoside chemical structure results in a binding both to the anionic ou...
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Protein conjugates of pyridoxal have been used to elicit anti-vitamin B6 antibodies in rabbits. These antibodies have been incorporated into 2 homogeneous assays systems, a spin immunoassay, using a paramagnetic derivative of the vitamin as ligand, and a fluorescence enzyme immunoassay, using beta-galactosidase conjugated to vitamin B6 as the indicator molecule. These assay systems do not requi...
متن کاملMultiple resistance to aminoglycoside antibiotics in actinomycetes.
Actinomycetes were characterized in terms of resistance to 11 different aminoglycoside antibiotics (AGs). Strains freshly isolated in AG containing media showed wide varieties of multiple AG resistance, while the majority of ISP (International Streptomyces Project) cultures and the actinomycete strains isolated in an AG free medium were susceptible to all or most of the AGs tested. Marked chara...
متن کاملBinding of aminoglycoside antibiotics to acidic mucopolysaccharides.
Binding of aminoglycoside antibiotics to acidic mucopolysaccharides has been studied by means of physicochemical methods. Reactivity was affected markedly by the ionic environment , e.g. pH and ionic strength of the medium, the concentrations and the molar ratios of the constituents. The ionic character of binding was further confirmed by gel chromatography. The reduction of metachromasis by an...
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ژورنال
عنوان ژورنال: Canadian Journal of Chemistry
سال: 1984
ISSN: 0008-4042,1480-3291
DOI: 10.1139/v84-425